Why is it necessary to deskew lattice light sheet data?
The fact that the detection objective lens is tilted at an angle with [...]
Preview images remotely
Using the llsinfo over an ssh connection, you can also preview lattice light sheet [...]
Getting info on an LLS data folder
When logged in to the CBMF server via ssh, you can use the [...]
Transferring files from the CBMF server
There are multiple way to transfer files from the CBMF server. Note all [...]
Combining multiple channels/timepoints into a hyperstack in Fiji
By default, data acquired on the lattice is saved as individual Z-stack TIFF [...]
Camera pixel correction
Understanding the Correction There is a phenomenon in the camera used on the [...]
Explanation of LLS data structure/format
Top level LLS folder A typical lattice light sheet experiment will yield a single [...]
Compressing and decompressing data for short and long-term storage
Short term storage We recommend compressing just the raw data after deskewing/deconvolving. This [...]
Post-processing without auto-cropping
By default, deskewed/deconvolved images will be “auto-cropped” to exclude regions that do not [...]
Retrieving deconvolved Z-stacks
By default, the lls command will generate a Deskewed folder that contains the complete [...]