Currently, our lattice has a single camera, and uses multi-band-pass filters to achieve fast multi-channel imaging. This sometimes comes at the expense of increased bleed through (when compared to a conventional microscope setup in which emission filters are in a filter wheel, and are changed depending on the laser ). You can use the tool below to see what filter sets we currently have on the lattice, and evaluate whether your fluorophore combination might suffer from bleed through. Pay particular attention to the excitation: for example, if your “red” fluorophore is partially excitated by the 488 laser (for example, look at mRFP1: it is excited with ~35% efficiency by the 488nm laser), then you will likely see bleedthrough of the red fluorophore in the “green” (488nm) channel image.
Lattice Filter Spectratalley2017-02-28T16:47:25-05:00